Oncology Application Notes and Publications

Deep central pulmonary metastases: detection and measurement of disease and therapeutic response (ProSense)

Fluorescence quantification corresponds to tumor burden as assessed by visual examination, lung weight and histological analysis

Treated
Treated
Untreated
Untreated
Image: Coronal slices taken at lung depth show a dramatic effect of treatment on tumor-related fluorescence.
Investigation: Four- to five- week old female BALB/c mice received a chemotherapeutic regimen of Doxycycline (15 mg/kg/d) beginning one day following 4T1 cell injection. On day 13, mice were injected with ProSense 750 and imaged 24 hours later.

Real-time imaging of the blood pool and vascularity associated with tumor mass (AngioSense)
FMT-based imaging of tumor using AngioSense
Enlarge
Tumor imaging using FMT System and AngioSense
Fluorescence microscopy reveals distribution of tumor cells (DAPI, blue) and AngioSense (red). Magnification x 100
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Fluorescence microscopy reveals distribution of tumor cells (DAPI, blue) and AngioSense (red). Magnification x 100
Image: Nude mouse implanted with PC3 human prostate cancer.
Investigation: After 15 days, mouse was injected with AngioSense (2 nmoles) and imaged 24 hours later by FMT System. Immediately after, tumor was excised, snap frozen and sectioned (5 µm).

Following therapeutic response
FMT System and ProSense
FMT System with ProSense
Protease activity in 4T1 breast cancer metastasis in the lung

Scanning for disseminated metastases – consistent with known histology
Analysis of femur and vertebral metastases using ProSense 750
Consistent pattern of tumor metastases in the femoral region
Consistent pattern of tumor metastases in spinal and pelvic bone region