Labeling agents

AminoSPARK nanoparticles and VivoTag fluorochromes enable development of novel superbright fluorescence agents with properties that are ideal for extended use in in vivo or in vitro imaging.

Excellent for in vitro and in vivo imaging

IVM, FMT, FRI or fluorescence microscopy

Provide superbright, stable fluorescence for longer times than previously possible

Ideal for in vivo imaging

minimal cytotoxicity, narrow size distribution, highly functionalized

Customizable targeting

			with amine-reactive molecules, such as NHS esters, anhydrides or EDC activated carboxylates
			with chosen fluorochrome

Minimal cytotoxicity

Viability of RAW 264 cells incubated with nanoparticles for 24 hrs (assessed using CellTiter-Glo Luminescent Cell Viability Assay).

Dose-dependent uptake of nanoparticles in cytoplasm and nucleus

FACS analysis of labeled RAW 264 cells after incubation with AminoSPARK 680 (1X and 10X concentration), derivatized with VivoTag 680, for 4 hrs and dual-channel fluorescence microscopy

Application examples

Arthritic mouse model: AminoSPARK 680 derivatized with VivoTag 680

A: Healthy, control

B: Arthritis -induced (intravenous injection with a collagen cocktail (4 mg/mouse, Chemicon), day 0, and intraperitoneal injection of lipopolysaccharide, (50 μg/ mouse), day 3

C: Dexamethasone-treated

Images: Male BALB/c mice (5 weeks old)
Investigation: Disease severity peaked at 8-9 days. Mice were injected intravenously with AminoSPARK 680 on day 7 and imaging took place after 24 hours.

Detailed application notes for download:

A New Platform of Superbright Fluorescent Nanoparticles for In Vivo Imaging (2006) (1.3 MB)

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