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Fluorescence nanoparticles for derivatization
AminoSPARK 680, AminoSpark 750 |
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Schematic of AminoSPARK derivatized with VivoTag 680
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For further details of the chemistry behind superbright fluorescence nanoparticles..
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AminoSPARK 680, AminoSPARK 750 are derivatizable, non-superparamagnetic, fluorescence nanoparticles (based on an iron oxide core), coated with a biocompatible polymer, and an amine-functionalized surface for further chemistry and conjugation.
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| AminoSPARK 680 |
| Absorbance, excitation and emission spectra in 1 x PBS |
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| Chemistry |
Iron oxide core, 5-10 nm diameter, c. 8000 Fe atoms, with non-crosslinked polymer coating |
| Particle size |
20-50 nm-1 |
| Fluorescence Excitation |
673 ± 5 nm |
| Fluorescence Emission |
690 ± 5 nm |
| Extinction |
3 x 106 ± 2 x 106 M-1cm-1 |
| Appearance |
Greenish-brown solution |
| AminoSPARK 750 |
| Absorbance, excitation and emission spectra in 1 x PBS |
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| Chemistry |
Iron oxide core, 5-10 nm diameter, c. 8000 Fe atoms, with non-crosslinked polymer coating |
| Particle size |
20-50 nm-1 |
| Fluorescence Excitation |
753 ± 5 nm |
| Fluorescence Emission |
773 ± 5 nm |
| Extinction |
3 x 106 ± 2 x 106 M-1cm-1 |
| Appearance |
Greenish-brown solution |
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Derivatization |
AminoSPARK 680 and AminoSPARK 750 contain an iron oxide core with an amine-functionalized surface suitable for further chemistry and conjugation to a variety of amine reactive molecules, such as NHS esters, anhydrides or EDC activated carboxylates, using standard amine coupling conditions.
| Product |
Supplied concentration* |
Recommended injection concentration after derivatization |
Recommended injection volume for an ~25 gram mouse |
| AminoSPARK 680 |
3 mg [Fe]/mL (6.7 μM, equivalent to 1600 nmol of reactive amines) |
3.3 μM or 150 μg [Fe] / 100μL in 1 x PBS |
100 μl |
| AminoSPARK 750 |
3 mg [Fe]/mL (6.7 μM, equivalent to 1600 nmol of reactive amines) |
3.3 μM or 150 μg [Fe] / 100μL in 1 x PBS |
100 μl |
* Supplied in 1 mL of 1 x PBS, sufficient for derivatization and subsequent imaging of up to 10 x ~25 gram mice.
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Administration of Fluorescence Agents |
Allow fluorescence agents to reach room temperature before injection.
Inject agents intravenously via the tail vein.
Use a small gauge (27G) needle and dispense slowly into the tail vein.
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Imaging Time Points |
The agent conjugated to the AminoSPARK nanoparticle will influence the distribution and in vivo imaging efficacy of the derivatized molecule.
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Fluorochrome tags
VivoTag-S 680, VivoTag-S 750 |
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VivoTag-S 680, VivoTag-S 750 are amine-reactive near-infra-red fluorochromes for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules.
| VivoTag-S 680 |
Absorbance and emission spectra in 1 x PBS.
(Arrow indicates shift in absorbance of conjugate with multiple fluorochromes). |
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| MW |
~1200 g mol-1 |
| Fluorescence Excitation |
673 ± 5 nm |
| Fluorescence Emission |
691 ± 5 nm |
| Absorbance |
673 ± 5 nm |
| Extinction |
~220,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 675 nm) |
>90% |
| Appearance |
Blue solid |
| VivoTag-S 750 |
Absorbance and emission spectra in 1 x PBS.
(Arrow indicates shift in absorbance of conjugate with multiple fluorochromes).
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| MW |
~980 g mol-1 |
| Fluorescence Excitation |
750 ± 5 nm |
| Fluorescence Emission |
775 ± 5 nm |
| Absorbance |
750 ± 5 nm |
| Extinction |
~240,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 750 nm) |
>90% |
| Appearance |
Blue solid |
VivoTag 680 |
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VivoTag 680 is an amine-reactive near-infra-red fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. This fluorochrome exhibits lower quenching than VivoTag-S 680.
| VivoTag 680 |
| Absorbance and emission spectra in 1 x PBS |
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| MW |
~1240 g mol-1 |
| Fluorescence Excitation |
670 ± 5 nm |
| Fluorescence Emission |
688 ± 5 nm |
| Absorbance |
670 ± 5 nm |
| Extinction |
~100,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 670 nm) |
>90% |
| Appearance |
Blue solid |
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Labeling of biomolecules or nanoparticles |
Allow VivoTag-S 680, VivoTag-S 750 and VivoTag 680 to reach room temperature before use.
Note that labeling of target molecules that have multiple amine reactive sites with >1 fluorochrome results in a blue shift in the absorbance spectra of VivoTag-S 680 and VivoTag-S 750. The emission spectra are unaffected.
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Administration of Fluorescence Agents |
Allow fluorescence agents to reach room temperature before injection.
Inject agents intravenously via the tail vein.
Use a small gauge (27G) needle and dispense slowly into the tail vein.
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Imaging Time Points |
The molecule or nanoparticle to which the tag has been conjugated will influence the distribution and in vivo imaging efficacy of the fluorescence agent.
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For additional information and application support, please contact us
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