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Fluorescence nanoparticles for derivatization
AminoSPARK 680, AminoSpark 750 |
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Schematic of AminoSPARK derivatized with VivoTag 680
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For further details of the chemistry behind superbright fluorescence nanoparticles..
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AminoSPARK 680, AminoSPARK 750 are derivatizable, non-superparamagnetic, fluorescence nanoparticles (based on an iron oxide core), coated with a biocompatible polymer, and an amine-functionalized surface for further chemistry and conjugation.
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| AminoSPARK 680 |
| Absorbance, excitation and emission spectra in 1 x PBS |
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| Chemistry |
Iron oxide core, 5-10 nm diameter, c. 8000 Fe atoms, with non-crosslinked polymer coating |
| Particle size |
20-50 nm-1 |
| Fluorescence Excitation |
673 ± 5 nm |
| Fluorescence Emission |
690 ± 5 nm |
| Extinction |
3 x 106 ± 2 x 106 M-1cm-1 |
| Appearance |
Greenish-brown solution |
| AminoSPARK 750 |
| Absorbance, excitation and emission spectra in 1 x PBS |
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| Chemistry |
Iron oxide core, 5-10 nm diameter, c. 8000 Fe atoms, with non-crosslinked polymer coating |
| Particle size |
20-50 nm-1 |
| Fluorescence Excitation |
753 ± 5 nm |
| Fluorescence Emission |
773 ± 5 nm |
| Extinction |
3 x 106 ± 2 x 106 M-1cm-1 |
| Appearance |
Greenish-brown solution |
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Derivatization |
AminoSPARK 680 and AminoSPARK 750 contain an iron oxide core with an amine-functionalized surface suitable for further chemistry and conjugation to a variety of amine reactive molecules, such as NHS esters, anhydrides or EDC activated carboxylates, using standard amine coupling conditions.
| Product |
Supplied concentration* |
Recommended injection concentration after derivatization |
Recommended injection volume for an ~25 gram mouse |
| AminoSPARK 680 |
3 mg [Fe]/mL (6.7 μM, equivalent to 1600 nmol of reactive amines) |
3.3 μM or 150 μg [Fe] / 100μL in 1 x PBS |
100 μl |
| AminoSPARK 750 |
3 mg [Fe]/mL (6.7 μM, equivalent to 1600 nmol of reactive amines) |
3.3 μM or 150 μg [Fe] / 100μL in 1 x PBS |
100 μl |
* Supplied in 1 mL of 1 x PBS, sufficient for derivatization and subsequent imaging of up to 10 x ~25 gram mice.
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Administration of Fluorescence Agents |
Allow fluorescence agents to reach room temperature before injection.
Inject agents intravenously via the tail vein.
Use a small gauge (27G) needle and dispense slowly into the tail vein.
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Imaging Time Points |
The agent conjugated to the AminoSPARK nanoparticle will influence the distribution and in vivo imaging efficacy of the derivatized molecule.
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Fluorochrome tags
VivoTag 645 |
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Part #
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Agent Description
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VivoTag 645 including 1 vial, 5 mg - Amine-reactive red fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, nanoparticles and macromolecules such as proteins and antibodies. |
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VivoTag 645 including 1 vial, 1 mg - Amine-reactive red fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, nanoparticles and macromolecules such as proteins and antibodies. |
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VivoTagTM 645 is an amine reactive red fluorochrome (NHS ester) for molecular labeling applications. VivoTag 645 enables optimized and integrated imaging applications in both in vivo and in vitro applications. VivoTag 645's superior brightness makes it particularly well suited for the labeling and imaging of proteins, antibodies, and small molecules in a wide range of research areas. The 645 nm fluorescence wavelength is ideal for biological imaging applications on in vitro and in vivo fluorescence systems, including advanced microscopes, high content imaging and flow cytometry systems. It is also optimized for use with VisEn's fluorescence tomography FMTTM imaging systems with expanded wavelength capabilities to enable quantitative imaging of up to four channels and four different biomarkers of interest in vivo.
| VivoTag 645 |
| Absorbance and emission spectra in 1 x PBS. |
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| MW |
1393 g mol-1 (MW as tris TEA Salt) |
| Fluorescence Excitation |
640 |
| Fluorescence Emission |
660 |
| Absorbance |
643 |
| Extinction |
~210,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 645 nm) |
>90% |
| Appearance |
Royal Blue solid |
VivoTag-S 680, VivoTag-S 750 |
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VivoTag-S 680, VivoTag-S 750 are amine-reactive near-infra-red fluorochromes for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules.
| VivoTag-S 680 |
Absorbance and emission spectra in 1 x PBS.
(Arrow indicates shift in absorbance of conjugate with multiple fluorochromes). |
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| MW |
~1200 g mol-1 |
| Fluorescence Excitation |
673 ± 5 nm |
| Fluorescence Emission |
691 ± 5 nm |
| Absorbance |
673 ± 5 nm |
| Extinction |
~220,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 675 nm) |
>90% |
| Appearance |
Blue solid |
| VivoTag-S 750 |
Absorbance and emission spectra in 1 x PBS.
(Arrow indicates shift in absorbance of conjugate with multiple fluorochromes).
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| MW |
~980 g mol-1 |
| Fluorescence Excitation |
750 ± 5 nm |
| Fluorescence Emission |
775 ± 5 nm |
| Absorbance |
750 ± 5 nm |
| Extinction |
~240,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 750 nm) |
>90% |
| Appearance |
Blue solid |
VivoTag 680 XL, VivoTag 680, VivoTag 800: |
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VivoTag 680 XL, 680 and 800 are amine-reactive near-infra-red fluorochromes for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies, nanoparticales or other macromolecules. This fluorochrome exhibits lower quenching than VivoTag-S family of dyes.
VivoTag® 680 XL |
| Absorbance and emission spectra in 1 x PBS |
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| MW |
1856 g mol-1 |
| Fluorescence Excitation |
665 ± 5 nm |
| Fluorescence Emission |
688 ± 5 nm |
| Absorbance |
668 ± 3 nm |
| Extinction |
~210,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 670 nm) |
>95% |
| Appearance |
Blue solid |
| VivoTag 680 |
| Absorbance and emission spectra in 1 x PBS |
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| MW |
~1240 g mol-1 |
| Fluorescence Excitation |
670 ± 5 nm |
| Fluorescence Emission |
688 ± 5 nm |
| Absorbance |
670 ± 5 nm |
| Extinction |
~100,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 670 nm) |
>90% |
| Appearance |
Blue solid |
| VivoTag 800 |
| Absorbance and emission spectra in 1 x PBS |
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| MW |
~1465 g mol-1 |
| Fluorescence Excitation |
785 nm |
| Fluorescence Emission |
810 nm |
| Absorbance |
787 ± 5 nm |
| Extinction |
~200,000 M-1cm-1 |
| Purity (reactive ester as determined by RP-HPLC, measuring absorbance at 790 nm) |
>90% |
| Appearance |
Green solid |
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Labeling of biomolecules or nanoparticles |
Allow Vivo Tag 645, VivoTag-S 680, VivoTag-S 750, VivoTag 680 XL, VivoTag 680 and VivoTag 800 to reach room temperature before use. Note that labeling of target molecules that have multiple amine reactive sites with >1 fluorochrome results in a blue shift in the absorbance spectra of VivoTag-S 680 and VivoTag-S 750. The emission spectra are unaffected.
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Administration of Fluorescence Agents |
Allow fluorescence agents to reach room temperature before injection.
Inject agents intravenously via the tail vein.
Use a small gauge (27G) needle and dispense slowly into the tail vein.
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Imaging Time Points |
The molecule or nanoparticle to which the tag has been conjugated will influence the distribution and in vivo imaging efficacy of the fluorescence agent.
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For additional information and application support, please contact us
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