Introduction Publications Technical Support Ordering MSDS

AngioSense 680, AngioSense-IVM 680
AngioSense 750, AngioSense-IVM 750
These fluorescence agents are large (250k MW) macromolecules that remain localized in the vasculature for extended periods of time (approximate half life in plasma 6 h) to facilitate imaging and to monitor angiogenesis. IVM formulations are optimized for intravital microscopy.
Healthy paw
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Healthy paw
Diseased paw
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Diseased paw
Image: In vivo confocal data images acquired using the same parameters: 637nm Laser Projections with a 10 μm step size (20 serial images).
Investigation: Arthritis was induced using an anti-collagen antibody cocktail followed 3 days later by LPS in Balb/c mice. Angiosense was injected on day 6 (early disease) and imaged on the IVM 20-25 min later.
AngioSense 680, AngioSense-IVM 680
AngioSense 680 absorbance and emission spectra in 1 x PBS
MW ~250,000 g mol-1
Fluorescence Excitation 680 ±10 nm
Fluorescence Emission 700 ±10 nm
Absorbance 680 ±10 nm
Purity (determined by SE-HPLC, measuring absorbance at 680 nm) >95%
Appearance Clear blue solution
AngioSense 750, AngioSense-IVM 750
AngioSense 750 absorbance and emission spectra in 1 x PBS
MW ~250,000 g mol-1
Fluorescence Excitation 750 ±10 nm
Fluorescence Emission 780 ±10 nm
Absorbance 750 ±10 nm
Purity (determined by SE-HPLC, measuring absorbance at 750 nm) >95%
Appearance Clear blue solution

AngioSPARK 680, AngioSpark 750
These agents are based on fluorescent nanoparticles (20-35 nm). With an approximate half-life in plasma of 20 h, they are well suited for prolonged monitoring of blood vessels and observing phenomena such as blood leakage in tumors, inflammation and angiogenesis.
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Excellent for in vitro and in vivo imaging using IVM, FMT, FRI, FACS and fluorescence microscopy
Work with superbright, stable fluorescence for longer times than previously possible
Ideal for in vivo imaging applications
biocompatible, minimal cytotoxicity, narrow size distribution, highly functionalized
AngioSPARK 680
AngioSPARK 680 absorbance, excitation and emission spectra in 1 x PBS
Particle size 20-50 nm
Fluorescence Excitation 673 ± 5 nm
Fluorescence Emission 690 ± 5 nm
Extinction 4 x 106 ± 2 x 106 M-1cm-1
Appearance Greenish-brown solution
AngioSPARK 750
AngioSPARK 750 absorbance, excitation and emission spectra in 1 x PBS
Particle size 20-50 nm
Fluorescence Excitation 750 ± 5 nm
Fluorescence Emission 775 ± 5 nm
Extinction 4 x 106 ± 2 x 106 M-1cm-1
Appearance Greenish-brown solution
Further details of the chemistry behind superbright fluorescence nanoparticles from VisEn 

Superhance 680
Superhance 680
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These are small molecule (1500 MW) fluorescence agents that enable imaging of vascular leakage and circulation including that associated with early oncologic and ophthalmologic lesions. Superhance binds to albumin and has a half-life in plasma of approximately 2 h.
Superhance 680
Superhance 680 absorbance and emission spectra in 1 x PBS
MW ~1540 g mol-1
Fluorescence Excitation 675 ± 5 nm
Fluorescence Emission 692 ± 5 nm
Absorbance 675 ± 5 nm
Purity (determined by RP-HPLC, measuring absorbance at 675 nm) >95%
Appearance Clear blue solution

Genhance 680
Genhance 680
Genhance 680 absorbance and emission spectra in 1 x PBS
MW ~1140 g mol-1
Fluorescence Excitation 670 ± 5 nm
Fluorescence Emission 688 ± 5 nm
Absorbance 670 ± 5 nm
Extinction ~100,000 M-1cm-1
Purity (determined by RP-HPLC, measuring absorbance at 670 nm) >90%
Appearance Blue solid

Genhance 750
Genhance 750
Genhance 750 absorbance and emission spectra in 1 x PBS
MW ~885 g mol-1
Fluorescence Excitation 750 ± 5 nm
Fluorescence Emission 775 ± 5 nm
Absorbance 750 ± 5 nm
Extinction ~100,000 M-1cm-1
Purity (determined by RP-HPLC, measuring absorbance at 750 nm) >90%
Appearance Blue solid
Fluorescence Agent Preparation
The products below are supplied at a concentration recommended for injection, no dilution is required:
Product Supplied/recommended injection concentration Recommended injection volume for an ~25 gram mouse
AngioSense 680 2 nmol/150 μl in 1 x PBS 150 μl
AngioSense 750 2 nmol/150 μl in 1 x PBS 150 μl
AngioSense-IVM 680 1000 μl in 1 x PBS 100 μl
AngioSense-IVM 750 1000 μl in 1 x PBS 100 μl
Superhance 680 4 nmol/150 μl in 1 x PBS 150 μl
AngioSPARK 680 500 μl in 1 x PBS 100 μl
AngioSPARK 750 500 μl in 1 x PBS 100 μl
The products below are supplied as solids for dissolution in 1 x PBS (not supplied):
Product Recommended injection concentration Recommended injection volume for an ~25 gram mouse
Genhance 680 0.05 mg/100 μl in 1 x PBS 100 μl
Genhance 750 0.05 mg/100 μl in 1 x PBS 100 μl
Administration of Fluorescence Agents
Allow fluorescence agents to reach room temperature before injection.
Inject intravenously via the tail vein.
Use a small gauge (27G) needle and dispense slowly into the tail vein.
Imaging Time Points
Fluorescence agent Recommend imaging time
post tail vein injection.
AngioSense 680 0 – 24 hours
AngioSense 750 0 – 24 hours
AngioSense-IVM 680 0 – 4 hours
AngioSense-IVM 750 0 – 4 hours
Superhance 680 0 – 24 hours
Genhance 680 0 – 24 hours
Genhance 750 0 – 24 hours
AngioSPARK 680 0 – 24 hours*
AngioSPARK 750 0 – 24 hours*
* Can be used for up to 12 hours later to image vascular leakage
in sites of tumors or inflammation. Can be imaged in the interstitium
for up to 24 hours post tail vein injection.
For additional information and application support, please contact us